7YCS
Heterotetramer of Antitoxin PrpA together with Toxin PrpT from Pseudoalteromonas rubra
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-16 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.782, 83.085, 56.489 |
| Unit cell angles | 90.00, 91.66, 90.00 |
Refinement procedure
| Resolution | 27.020 - 1.790 |
| R-factor | 0.1722 |
| Rwork | 0.171 |
| R-free | 0.19510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.918 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.020 | 1.857 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.084 | 0.791 |
| Rmeas | 0.091 | 0.872 |
| Rpim | 0.035 | 0.359 |
| Number of reflections | 38255 | 3738 |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 98.6 | 97.22 |
| Redundancy | 6.7 | |
| CC(1/2) | 0.999 | 0.809 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 0.1 M Sodium cacodylate pH 6.0, 15 % w/v PEG 4000 |
