7YCJ
Crystal structure of Vac8 bound to Vac17
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-05-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9919 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 147.446, 147.446, 121.514 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.862 - 2.101 |
R-factor | 0.1893 |
Rwork | 0.188 |
R-free | 0.22330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xjg |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 38976 | 38976 |
<I/σ(I)> | 27.2 | |
Completeness [%] | 99.6 | |
Redundancy | 9.5 | |
CC(1/2) | 0.999 | 0.365 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 25% (w/v) SOKALAN cp42, 100 mM, Tris-HCl pH 8.5, 100 mM sarcosine |