7YCJ

Crystal structure of Vac8 bound to Vac17

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-05-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9919
Spacegroup name	I 4 2 2
Unit cell lengths	147.446, 147.446, 121.514
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.862 - 2.101
R-factor	0.1893
Rwork	0.188
R-free	0.22330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xjg
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	38976	38976
<I/σ(I)>	27.2
Completeness [%]	99.6
Redundancy	9.5
CC(1/2)	0.999	0.365

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	25% (w/v) SOKALAN cp42, 100 mM, Tris-HCl pH 8.5, 100 mM sarcosine