7YC1
Crystal structure of FGFR4 kinase domain with 10d
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2022-05-31 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5870 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.153, 60.977, 60.488 |
| Unit cell angles | 90.00, 97.82, 90.00 |
Refinement procedure
| Resolution | 42.741 - 2.535 |
| R-factor | 0.1851 |
| Rwork | 0.183 |
| R-free | 0.22590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wct |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.639 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.741 | 2.626 |
| High resolution limit [Å] | 2.535 | 2.535 |
| Rmeas | 0.222 | |
| Number of reflections | 9570 | 631 |
| <I/σ(I)> | 8.52 | 2.88 |
| Completeness [%] | 93.8 | 62.05 |
| Redundancy | 6.3 | 2.7 |
| CC(1/2) | 0.982 | 0.897 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 0.1 M Bis-Tris (pH 4.5), 0.2 M Li2SO4, 16-19 % PEG 3350 |
