7YBP
Crystal structure of FGFR4(V550L) kinase domain with 10z
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2022-05-17 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5870 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.253, 61.188, 60.611 |
| Unit cell angles | 90.00, 98.05, 90.00 |
Refinement procedure
| Resolution | 42.845 - 2.243 |
| R-factor | 0.1782 |
| Rwork | 0.176 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wct |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.724 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.845 | 2.323 |
| High resolution limit [Å] | 2.243 | 2.243 |
| Rmerge | 0.196 | 0.292 |
| Rmeas | 0.211 | |
| Number of reflections | 14441 | 1317 |
| <I/σ(I)> | 8.98 | 4.88 |
| Completeness [%] | 97.7 | 90.08 |
| Redundancy | 6.9 | 5.1 |
| CC(1/2) | 0.983 | 0.938 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 0.1 M Bis-Tris (pH 4.5), 0.2 M Li2SO4, 16-19 % PEG 3350 |
