7YBO

Crystal structure of FGFR4 kinase domain with 10z

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2022-04-28
Detector	RIGAKU
Wavelength(s)	1.5870
Spacegroup name	P 1 21 1
Unit cell lengths	40.075, 60.440, 59.899
Unit cell angles	90.00, 94.93, 90.00

Refinement procedure

Resolution	42.465 - 2.307
R-factor	0.2094
Rwork	0.207
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wct
RMSD bond length	0.003
RMSD bond angle	0.776
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.470	2.389
High resolution limit [Å]	2.307	2.307
Rmerge	0.173	0.288
Rmeas	0.187
Number of reflections	11880	769
<I/σ(I)>	9.03	3.8
Completeness [%]	93.6	61.52
Redundancy	6.5	4
CC(1/2)	0.989	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277	0.1 M Bis-Tris (pH 4.5), 0.2 M Li2SO4, 16-19 % PEG 3350