7YBF

Crystal structure of inner membrane protein Sad1 in complex with histone H2A-H2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	42.804, 61.981, 76.016
Unit cell angles	90.00, 101.89, 90.00

Refinement procedure

Resolution	37.190 - 2.150
R-factor	0.2028
Rwork	0.198
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wnn
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.230
High resolution limit [Å]	2.150	4.630	2.150
Rmerge	0.123	0.086	0.552
Rmeas	0.136	0.096	0.610
Rpim	0.057	0.041	0.256
Total number of observations	118345
Number of reflections	21380	2201	2114
<I/σ(I)>	5.6
Completeness [%]	99.8	99.5	99.7
Redundancy	5.5	5.4	5.5
CC(1/2)		0.991	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M Potassium nitrate, 20% w/v Polyethylene glycol 3350