7YAQ
Phloem lectin (PP2)C34S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2022-01-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 51.970, 51.970, 261.521 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.395 - 1.853 |
| Rwork | 0.264 |
| R-free | 0.31620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vub |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.774 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.010 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.210 | 0.248 |
| Number of reflections | 18583 | 2563 |
| <I/σ(I)> | 6.2 | 4.3 |
| Completeness [%] | 98.7 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.944 | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 295 | 18% 1,4 butanediol, 300 mM Zinc acetate and 0.1 M Imidazole-HCl pH 6-7 |
