7YA9

Fis1 (Mitochondrial fission 1 protein)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	56.066, 56.066, 97.661
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.640 - 1.700
R-factor	0.1892
Rwork	0.188
R-free	0.22150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nzn
RMSD bond length	0.018
RMSD bond angle	1.562
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.074	0.969
Number of reflections	17973	872
<I/σ(I)>	25.2
Completeness [%]	99.9	99.9
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.1 M CAPS pH 10.5 30% PEG 400