7YA4
Formate dehydrogenase from Novosphingobium sp. AP12 with NAD and Azide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-03-06 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.794, 95.789, 117.930 |
| Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
| Resolution | 34.138 - 1.800 |
| Rwork | 0.155 |
| R-free | 0.19080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xyb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.533 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.287 |
| Number of reflections | 143534 | 6878 |
| <I/σ(I)> | 24.6 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | PEG 3350, Tacsimate (Hampton Research, USA) |
