7Y9I

Complex structure of AtYchF1 with ppGpp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	113
Detector technology	PIXEL
Collection date	2019-03-24
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	72.460, 111.500, 52.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.005 - 2.070
Rwork	0.206
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ee0
RMSD bond length	0.006
RMSD bond angle	1.425
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.120
High resolution limit [Å]	2.070	2.070
Rmerge	0.073
Rpim	0.028	0.392
Number of reflections	26569	1922
<I/σ(I)>	18.8
Completeness [%]	99.9
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	277	PEG