7Y9A
Crystal structure of sDscam Ig1-2 domains, isoform beta2v6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 63.256, 49.018, 82.024 |
| Unit cell angles | 90.00, 104.66, 90.00 |
Refinement procedure
| Resolution | 26.880 - 2.510 |
| R-factor | 0.2432 |
| Rwork | 0.241 |
| R-free | 0.27850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dmk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.081 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.087 | 0.068 | 0.211 |
| Rmeas | 0.095 | 0.075 | 0.234 |
| Rpim | 0.038 | 0.029 | 0.099 |
| Total number of observations | 50140 | ||
| Number of reflections | 8315 | 448 | 374 |
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 98.8 | 99.8 | 89.7 |
| Redundancy | 6 | 6.1 | 5.1 |
| CC(1/2) | 0.971 | 0.996 | 0.973 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M magnesium chloride, 0.1 M HEPES pH7.0, 20 % (w/v) PEG 6,000 |
