7Y95

Crystal structure of sDscam Ig1 domain, isoform beta6v2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	45.177, 39.096, 60.873
Unit cell angles	90.00, 108.97, 90.00

Refinement procedure

Resolution	19.548 - 1.550
R-factor	0.1521
Rwork	0.151
R-free	0.19130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zr7
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.580
High resolution limit [Å]	1.550	4.210	1.550
Rmerge		0.031	0.609
Rmeas		0.034	0.695
Rpim		0.013	0.327
Number of reflections	30014	1547	1472
<I/σ(I)>	22.5
Completeness [%]	99.6	99.3	97.9
Redundancy	5.7	6	4.1
CC(1/2)	0.991	0.996	0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M Magnesium Formate, 20% (w/v) PEG3,350