7Y8W

Crystal structure of DLC-1/SAO-1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	40.274, 163.515, 229.132
Unit cell angles	90.00, 90.46, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.2544
Rwork	0.253
R-free	0.28490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p8m
RMSD bond length	0.003
RMSD bond angle	0.707
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.129	0.058	0.762
Rmeas	0.142	0.064	0.847
Rpim	0.059	0.026	0.361
Total number of observations	285979
Number of reflections	55428	2653	2782
<I/σ(I)>	3.9
Completeness [%]	95.3	89.7	97
Redundancy	5.2	5.2	4.9
CC(1/2)		0.996	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.2 M Potassium chloride, 0.05 M HEPES pH 7.5, 35% (v/v) Pentaerythritol propoxylate