7Y8U

Crystal structure of AlbEF homolog from Quasibacillus thermotolerans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2018-12-15
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00000
Spacegroup name	P 31 2 1
Unit cell lengths	136.927, 136.927, 118.353
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.920 - 2.100
R-factor	0.2044
Rwork	0.203
R-free	0.22740
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.384
Data reduction software	XDS (20180808)
Data scaling software	Aimless (0.7.3)
Phasing software	PHENIX (1.11.1_2575)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.820	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.057	0.926
Rmeas	0.057	0.940
Rpim	0.010	0.165
Number of reflections	74554	4535
<I/σ(I)>	44.6	5.7
Completeness [%]	99.6	98.9
Redundancy	30.9	31.8
CC(1/2)	1.000	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	sodium/potassium phosphate