7Y7U

Dimeric structure of a Quorum-Quenching metallo-hydrolase, LrsL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 41 3 2
Unit cell lengths	166.510, 166.510, 166.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.180 - 1.890
Rwork	0.191
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p9e
RMSD bond length	0.011
RMSD bond angle	1.719
Data reduction software	XDS
Data scaling software	XDS
Phasing software	BALBES
Refinement software	REFMAC (5.8.0349)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.180	1.958
High resolution limit [Å]	1.890	1.890
Rmerge	0.154	6.561
Rmeas	0.155	6.601
Rpim	0.017	0.719
Number of reflections	63574	6220
<I/σ(I)>	31.63	0.71
Completeness [%]	100.0	99.98
Redundancy	81.3	83.6
CC(1/2)	1.000	0.368

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1.6 M tri-Sodium citrate tribasic