7Y7Q

QDE-1 in complex with RNA template, RNA primer and 3'-dGTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-10
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97928
Spacegroup name	P 1
Unit cell lengths	81.211, 85.813, 101.581
Unit cell angles	102.56, 104.05, 94.06

Refinement procedure

Resolution	29.310 - 2.050
R-factor	0.1976
Rwork	0.196
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j7n
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.120
High resolution limit [Å]	2.050	4.410	2.050
Rmerge	0.081	0.043	0.525
Rmeas	0.096	0.051	0.659
Rpim	0.052	0.026	0.392
Total number of observations	455234
Number of reflections	151259	15309	14134
<I/σ(I)>	14.6
Completeness [%]	94.5	95.7	88
Redundancy	3	3.5	2.2
CC(1/2)		0.996	0.368

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	291	PEG 4000, 200 mM KCl