7Y7N

Crystal structure of DUF371 domain-containing protein from Methanobrevibacter ruminantium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97919
Spacegroup name	P 41 21 2
Unit cell lengths	47.354, 47.354, 135.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.716 - 1.764
Rwork	0.224
R-free	0.26930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cbn
RMSD bond length	0.011
RMSD bond angle	1.729
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.720	1.790
High resolution limit [Å]	1.760	1.760
Rmerge	0.090	1.597
Rmeas	0.100	0.098
Rpim	0.043	0.763
Number of reflections	16164	864
<I/σ(I)>	12.3
Completeness [%]	100.0
Redundancy	9.3	9.8
CC(1/2)	0.999	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	12.00% w/v Polyethylene glycol 8,000,5.00% w/v Glycerol,100 mM Potassium chloride