7Y74

Apostichopus japonicus ferritin mutant-D129A/E132A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97892
Spacegroup name	C 1 2 1
Unit cell lengths	192.830, 130.950, 122.620
Unit cell angles	90.00, 119.40, 90.00

Refinement procedure

Resolution	47.910 - 1.980
R-factor	0.1783
Rwork	0.176
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wpn
RMSD bond length	0.013
RMSD bond angle	1.554
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.010
High resolution limit [Å]	1.980	5.370	1.980
Rmerge	0.054	0.029	0.402
Rmeas	0.064	0.035	0.484
Rpim	0.034	0.019	0.266
Total number of observations	617526
Number of reflections	182173	9279	8307
<I/σ(I)>	8.6
Completeness [%]	99.0	98.9	90.7
Redundancy	3.4	3.4	3.1
CC(1/2)		0.997	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.05 M Cadmium sulfate hydrate, 0.1 M HEPES pH 7.5, 1.0 M Sodium acetate trihydrate