7Y74
Apostichopus japonicus ferritin mutant-D129A/E132A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97892 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.830, 130.950, 122.620 |
| Unit cell angles | 90.00, 119.40, 90.00 |
Refinement procedure
| Resolution | 47.910 - 1.980 |
| R-factor | 0.1783 |
| Rwork | 0.176 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wpn |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.554 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.010 |
| High resolution limit [Å] | 1.980 | 5.370 | 1.980 |
| Rmerge | 0.054 | 0.029 | 0.402 |
| Rmeas | 0.064 | 0.035 | 0.484 |
| Rpim | 0.034 | 0.019 | 0.266 |
| Total number of observations | 617526 | ||
| Number of reflections | 182173 | 9279 | 8307 |
| <I/σ(I)> | 8.6 | ||
| Completeness [%] | 99.0 | 98.9 | 90.7 |
| Redundancy | 3.4 | 3.4 | 3.1 |
| CC(1/2) | 0.997 | 0.865 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.05 M Cadmium sulfate hydrate, 0.1 M HEPES pH 7.5, 1.0 M Sodium acetate trihydrate |
