7Y73
Crystal structure of sDscam Ig1 domain, isoform beta3v7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.649, 40.950, 54.231 |
| Unit cell angles | 90.00, 100.11, 90.00 |
Refinement procedure
| Resolution | 39.030 - 1.320 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zr7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.786 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.360 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Number of reflections | 39096 | 2249 |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 98.1 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.980 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M MgCl2, 0.1 M TRIS pH8.5, 20%(w/v) PEG 4,000 |
