7Y70

RLGSGG-AtPRT6 UBR box (P4332)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	193
Detector technology	PIXEL
Collection date	2019-11-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 43 3 2
Unit cell lengths	95.254, 95.254, 95.254
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.887 - 1.801
R-factor	0.2112
Rwork	0.210
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lhn
RMSD bond length	0.011
RMSD bond angle	1.230
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.887	1.865
High resolution limit [Å]	1.801	1.801
Number of reflections	14240	1389
<I/σ(I)>	45.857
Completeness [%]	99.9
Redundancy	49.6
CC(1/2)	1.000	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	1.5 M Ammonium sulfate, 0.1 M Tris pH 8.5, 12% (w/v) Glycerol