7Y6O

Crystal structure of sDscam Ig1-3 domains, isoform alpha25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97890
Spacegroup name	H 3 2
Unit cell lengths	117.484, 117.484, 171.494
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.915 - 3.102
R-factor	0.2529
Rwork	0.251
R-free	0.28740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zr7
RMSD bond length	0.004
RMSD bond angle	0.763
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.170
High resolution limit [Å]	3.100	7.640	3.100
Rmerge	0.114	0.088	0.917
Rmeas	0.118	0.091	0.957
Rpim	0.030	0.024	0.261
Total number of observations	135929
Number of reflections	8498	619	565
<I/σ(I)>	6.6
Completeness [%]	100.0	99.8	99.6
Redundancy	16	14.7	12.2
CC(1/2)	0.985	0.996	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M KSCN, 15% (w/v) PEG 2,000, 0.1 M Glycine, 0.1 M Bis-Tris pH6.0