7Y6O
Crystal structure of sDscam Ig1-3 domains, isoform alpha25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97890 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 117.484, 117.484, 171.494 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.915 - 3.102 |
| R-factor | 0.2529 |
| Rwork | 0.251 |
| R-free | 0.28740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zr7 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.763 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.170 |
| High resolution limit [Å] | 3.100 | 7.640 | 3.100 |
| Rmerge | 0.114 | 0.088 | 0.917 |
| Rmeas | 0.118 | 0.091 | 0.957 |
| Rpim | 0.030 | 0.024 | 0.261 |
| Total number of observations | 135929 | ||
| Number of reflections | 8498 | 619 | 565 |
| <I/σ(I)> | 6.6 | ||
| Completeness [%] | 100.0 | 99.8 | 99.6 |
| Redundancy | 16 | 14.7 | 12.2 |
| CC(1/2) | 0.985 | 0.996 | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M KSCN, 15% (w/v) PEG 2,000, 0.1 M Glycine, 0.1 M Bis-Tris pH6.0 |
