Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2019-03-19 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.057, 78.778, 113.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.051 - 2.703 |
| R-factor | 0.1928 |
| Rwork | 0.189 |
| R-free | 0.26170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6f9m |
| Data reduction software | HKL-3000 (v714n) |
| Data scaling software | HKL-3000 (v714n) |
| Phasing software | HKL-3000 (v714n) |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 7.310 | 2.700 |
| Rmerge | 0.089 | 0.029 | 0.421 |
| Rmeas | 0.107 | 0.036 | 0.522 |
| Rpim | 0.059 | 0.020 | 0.303 |
| Total number of observations | 48642 | ||
| Number of reflections | 16072 | 854 | 771 |
| <I/σ(I)> | 8.3 | ||
| Completeness [%] | 98.6 | 93.2 | 97.7 |
| Redundancy | 3 | 2.8 | 2.7 |
| CC(1/2) | 0.998 | 0.724 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277.15 | PROTEIN: 25 mM Tris-HCl, 50 mM Nacl, 1 mM CaCl2 MOTHER LIQUOR: 25% PEG3350, 8% Tacsimate pH 4.5 |
