7Y6E

Crystal structure of sDscam FNIII23 domains, isoform Beta2v6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97852
Spacegroup name	C 1 2 1
Unit cell lengths	307.021, 56.219, 94.837
Unit cell angles	90.00, 105.38, 90.00

Refinement procedure

Resolution	48.847 - 3.034
R-factor	0.2365
Rwork	0.234
R-free	0.27240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.644
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.100
High resolution limit [Å]	3.034	8.270	3.050
Rmerge	0.143	0.096	0.762
Rmeas	0.161	0.109	0.881
Rpim	0.072	0.049	0.433
Number of reflections	30260	1607	1376
<I/σ(I)>	5.2
Completeness [%]	98.7	98.1	90.9
Redundancy	4.7	4.7	3.8
CC(1/2)	0.989	0.985	0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M NaCl, 14% (w/v) PEG 8,000, 100 mM HEPES buffer pH 7.0