7Y6A

Crystal structure of Chicken Egg Lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-14
Detector	RIGAKU HyPix-3000
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	77.350, 77.350, 38.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.460 - 1.500
R-factor	0.2231
Rwork	0.222
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ijv
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHENIX
Refinement software	PHENIX (dev_3318)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.460	1.780
High resolution limit [Å]	1.500	1.500
Rmeas	0.080	0.328
Number of reflections	19182
<I/σ(I)>	15
Completeness [%]	98.9
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		294	0.09 M HEPES-NaOH pH 7.5, 1.26 M sodium citrate tribasic dihydrate, 10% v/v glycerol