7Y5Y

X-ray Structure of Stay-Green (SGR) from Anaerolineae bacterium.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.90000
Spacegroup name	P 61 2 2
Unit cell lengths	80.331, 80.331, 224.803
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.770 - 1.850
R-factor	0.2187
Rwork	0.218
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold2 predicted structure of rice SGR (UniProt: Q652K1)
RMSD bond length	0.009
RMSD bond angle	1.328
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.740	1.840
High resolution limit [Å]	1.750	1.750
Rmerge	0.061	1.158
Number of reflections	44094	6906
<I/σ(I)>	14.07
Completeness [%]	99.4
Redundancy	6.5
CC(1/2)	0.999	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	100 mM Tris-HCl (pH 8.5), 200 mM Ammonium phosphate monobasic and 50% (v/v) 2-Methyl-2,4-pentanediol