7Y5R

Crystal structure of sDscam FNIII2 domain, isoform alpha7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	28.853, 53.280, 60.817
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.640 - 1.562
R-factor	0.1398
Rwork	0.139
R-free	0.15880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1va9
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	1.730	1.600
High resolution limit [Å]	1.560	1.680	1.560
Rmerge	0.173	0.354	0.405
Rmeas	0.181	0.370	0.427
Rpim	0.051	0.108	0.134
Number of reflections	13917	884	917
<I/σ(I)>	13.9
Completeness [%]	100.0	100	100
Redundancy	11.9	11.4	9.7
CC(1/2)		0.965	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M HEPES sodium pH 7.5, 1.5 M Lithium sulfate monohydrate