7Y5M

The OTU domain of Tacheng Tick Virus 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	47.747, 58.110, 59.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.750 - 1.500
R-factor	0.1993
Rwork	0.198
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3phu
RMSD bond length	0.006
RMSD bond angle	0.895
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	27019	2598
<I/σ(I)>	15.3
Completeness [%]	99.5
Redundancy	6.2
CC(1/2)	0.976	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	PEG 4000,Lithium sulfate monohydrate,MES