7Y5J

Crystal structure of sDscam Ig1 domain, isoform alpha1v7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 65 2 2
Unit cell lengths	64.773, 64.773, 113.046
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.202 - 1.600
R-factor	0.1903
Rwork	0.189
R-free	0.21410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zr7
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.092	0.065	1.016
Rmeas	0.094	0.066	1.031
Rpim	0.015	0.011	0.171
Total number of observations	727817
Number of reflections	19264	1114	940
<I/σ(I)>	6
Completeness [%]	100.0	99.8	100
Redundancy	37.8	33.4	35.9
CC(1/2)		0.999	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	1 M Sodium citrate tribasic, 100 mM Sodium cacodylate/ Hydrochloric acid pH6.5