7Y54

Crystal structure of sDscam Ig1 domain, isoform alpha1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	67.395, 40.728, 41.482
Unit cell angles	90.00, 94.18, 90.00

Refinement procedure

Resolution	20.686 - 1.787
R-factor	0.1827
Rwork	0.180
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zr7
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.787	1.787
Number of reflections	10665	508
<I/σ(I)>	8.6
Completeness [%]	100.0
Redundancy	6.4
CC(1/2)	0.960	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	40% isopropanol, 100 mM Imidazole pH 6.5, 15% PEG 8000