7Y52

Crystal structure of peptidyl-tRNA hydrolase from Enterococcus faecium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2022-03-29
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	38.821, 74.835, 63.751
Unit cell angles	90.00, 104.85, 90.00

Refinement procedure

Resolution	23.780 - 1.920
R-factor	0.1865
Rwork	0.184
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yly
RMSD bond length	0.013
RMSD bond angle	1.278
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	1.990
High resolution limit [Å]	1.920	4.130	1.920
Rmerge	0.057	0.033	0.292
Rmeas	0.061	0.036	0.316
Rpim	0.023	0.013	0.118
Total number of observations	193162
Number of reflections	26521	2710	2617
<I/σ(I)>	15.4
Completeness [%]	100.0	99.6	100
Redundancy	7.3	7.4	7.1
CC(1/2)		0.999	0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.2 M Ammonium Sulfate, 0.1 M Tris-HCl pH 7.5, 15% PEG 4K