7Y4P

Human Plexin A1, extracellular domains 1-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-18
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9800
Spacegroup name	I 4 2 2
Unit cell lengths	224.774, 224.774, 205.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.280 - 3.500
R-factor	0.2218
Rwork	0.221
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3okt
RMSD bond length	0.004
RMSD bond angle	0.752
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.280	3.670
High resolution limit [Å]	3.500	3.500
Rmerge	0.105
Number of reflections	66184	4366
<I/σ(I)>	13.6	3
Completeness [%]	99.4
Redundancy	8.3
CC(1/2)	0.998	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	293	1.2 M sodium potassium phosphate buffer (pH 7.7)