7Y4H
AcvX from Actinomadura viridis that exhibits deglycosylation activity on lobophorins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-11 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54184 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 65.588, 66.605, 153.585 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.800 |
| R-factor | 0.1703 |
| Rwork | 0.169 |
| R-free | 0.20090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | model predicted by AlphaFold 2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.216 |
| Data reduction software | CrysalisPro (39.7e) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.452 |
| Number of reflections | 59112 | 1812 |
| <I/σ(I)> | 27.2 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.3 | 3.8 |
| CC(1/2) | 0.999 | 0.747 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.2 M ammonium acetate, 0.1 M Bis-Tris and 25% w/v PEG 3350 |
