7Y4F
bacterial DPP4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9783 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.800, 148.857, 137.125 |
| Unit cell angles | 90.00, 104.02, 90.00 |
Refinement procedure
| Resolution | 46.364 - 1.918 |
| R-factor | 0.1691 |
| Rwork | 0.168 |
| R-free | 0.19330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r9n |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.364 | 1.950 |
| High resolution limit [Å] | 1.918 | 1.920 |
| Number of reflections | 283945 | 3107 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.984 | 0.512 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291.15 | 0.1M BICINE pH 9.0, 10% w/v PEG 20,000 and 3% 6-aminohexanoic acid solution |
