7Y4C
Crystal structure of DUSP10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-12-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.235, 56.128, 69.594 |
| Unit cell angles | 92.01, 92.28, 105.28 |
Refinement procedure
| Resolution | 24.380 - 1.870 |
| R-factor | 0.2204 |
| Rwork | 0.218 |
| R-free | 0.26750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zzw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.102 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.045 | 0.178 |
| Number of reflections | 49102 | 48593 |
| <I/σ(I)> | 10.591 | 3.852 |
| Completeness [%] | 96.6 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 283 | 25% (w/v) PEG 3000, 100 mM Tris base /Hydrochloric acid, 175 mM Calcium acetate |
