7Y4B
Crystal structure of DUSP10 mutant_D59A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-02-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.230, 41.584, 56.826 |
| Unit cell angles | 96.03, 100.44, 117.45 |
Refinement procedure
| Resolution | 24.760 - 1.860 |
| R-factor | 0.1876 |
| Rwork | 0.184 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zzw |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.229 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.940 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.028 | 0.121 |
| Number of reflections | 23176 | 23130 |
| <I/σ(I)> | 27.748 | 6.65 |
| Completeness [%] | 88.8 | 88.3 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 22.5%(w/v) PEG 3000, 100mM Tris base/Hydrochloric acid, 200mM Calcium acetate |
