7Y3Y
Crystal structure of BTG13 mutant (T299V)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.310, 99.558, 129.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.990 - 1.920 |
| R-factor | 0.1717 |
| Rwork | 0.170 |
| R-free | 0.20050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7y3w |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.575 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 78.990 | 78.990 | 2.040 |
| High resolution limit [Å] | 1.920 | 5.770 | 1.920 |
| Rmerge | 0.105 | 0.036 | 0.617 |
| Rmeas | 0.112 | 0.039 | 0.664 |
| Rpim | 0.039 | 0.014 | 0.238 |
| Total number of observations | 610127 | 23504 | 87732 |
| Number of reflections | 77631 | 3142 | 12098 |
| <I/σ(I)> | 14.8 | 39.8 | 2.9 |
| Completeness [%] | 99.0 | 99.5 | 96.9 |
| Redundancy | 7.9 | 7.5 | 7.3 |
| CC(1/2) | 0.998 | 0.999 | 0.919 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.1 M Bis-Tris pH 6.45, 16% PEG10000 |
