7Y3P
Salmonella typhimurium YbiB protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-11 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 69.770, 69.770, 140.082 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.880 - 2.340 |
| R-factor | 0.2442 |
| Rwork | 0.242 |
| R-free | 0.28520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4muo |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.522 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.420 | 2.470 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.157 | 0.867 |
| Number of reflections | 16356 | 2416 |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 97.5 | |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292.15 | 0.1 M Sodium acetate trihydrate pH 4.5, 3.0 M Sodium chloride. |
