7Y3L

Structure of SALL3 ZFC4 bound with 12 bp AT-rich dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	111.549, 39.422, 37.986
Unit cell angles	90.00, 103.20, 90.00

Refinement procedure

Resolution	37.056 - 2.500
R-factor	0.2197
Rwork	0.217
R-free	0.26040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7y3i
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.060	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	5606	644
<I/σ(I)>	13
Completeness [%]	98.4
Redundancy	6.1
CC(1/2)	0.997	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M MES monohydrate, pH 6.5, 0.2 M Ammonium sulfate, 30% w/v polyethylene glycol monomethyl ether 5000