7Y3K

Structure of SALL4 ZFC4 bound with 16 bp AT-rich dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	43.364, 49.868, 105.406
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.722 - 2.501
R-factor	0.2103
Rwork	0.206
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7y3i
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	8329	8329
<I/σ(I)>	19
Completeness [%]	99.6
Redundancy	10.1
CC(1/2)	0.969	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.1 M BIS-TRIS, pH 5.5, 0.2 M Sodium chloride, 25% polyethylene glycol 3350