7Y3J

24B3 antibody-peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-27
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	49.589, 127.883, 160.342
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.230 - 2.600
R-factor	0.2239
Rwork	0.222
R-free	0.26110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bkc
RMSD bond length	0.002
RMSD bond angle	0.493
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.640
High resolution limit [Å]	2.600	2.600
Rmeas	0.097	0.415
Number of reflections	16218	805
<I/σ(I)>	32.1	8.6
Completeness [%]	100.0
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG3,350