7Y3I

Structure of DNA bound SALL4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	97
Detector technology	PIXEL
Collection date	2021-06-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	48.975, 69.197, 110.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.127 - 2.450
R-factor	0.1966
Rwork	0.194
R-free	0.24220
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.980	2.630
High resolution limit [Å]	2.450	2.450
Number of reflections	14297	14297
<I/σ(I)>	9.9
Completeness [%]	99.8
Redundancy	13.2
CC(1/2)	0.996	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M BIS-tris, pH 6.5, 25% w/v polyethylene glycol 3350