7Y37
Structure of a triple-helix region of human collagen type V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2022-05-31 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.865, 23.675, 36.648 |
| Unit cell angles | 90.00, 100.19, 90.00 |
Refinement procedure
| Resolution | 26.530 - 1.450 |
| R-factor | 0.1572 |
| Rwork | 0.156 |
| R-free | 0.18280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f01 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.8) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.530 | 26.530 | 1.470 |
| High resolution limit [Å] | 1.450 | 7.800 | 1.450 |
| Rmerge | 0.047 | 0.025 | 0.226 |
| Rmeas | 0.051 | 0.028 | 0.278 |
| Rpim | 0.020 | 0.012 | 0.159 |
| Total number of observations | 55181 | 432 | 258 |
| Number of reflections | 8727 | 75 | 85 |
| <I/σ(I)> | 28.3 | 34.3 | 7.6 |
| Completeness [%] | 86.1 | 95.9 | 16.5 |
| Redundancy | 6.3 | 5.8 | 3 |
| CC(1/2) | 0.998 | 1.000 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Ammonium Citrate Dibasic, 20% (w/v) PEG 3350 |
