7Y1U
Crystal structure of isocitrate dehydrogenase from Campylobacter corcagiensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.18067 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.501, 92.694, 147.321 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.930 - 2.500 |
| R-factor | 0.1919 |
| Rwork | 0.188 |
| R-free | 0.25890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1itw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.724 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.110 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.125 | 0.978 |
| Rpim | 0.036 | 0.272 |
| Number of reflections | 30370 | 3368 |
| <I/σ(I)> | 18.3 | 3.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 13.1 | |
| CC(1/2) | 0.999 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M Li2SO4, 0.1 M Tris pH8.5, 40% PEG400 |
