7Y19

Transcriptional regulator BrpR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100.15
Detector technology	PIXEL
Collection date	2021-07-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0448
Spacegroup name	P 61 2 2
Unit cell lengths	53.259, 53.259, 143.926
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.250 - 2.390
R-factor	0.2275
Rwork	0.221
R-free	0.29080
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.390	6.510	2.400
Rmerge	0.106	0.066	0.309
Rmeas	0.107	0.067	0.321
Rpim	0.019	0.012	0.082
Number of reflections	5217	326	252
<I/σ(I)>	17.2
Completeness [%]	98.6	95.9	96.9
Redundancy	24.2	29.9	12.8
CC(1/2)		0.999	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287.15	0.035 M Citric acid, 0.065 M BIS-TRIS propane pH 7.0, 22% w/v Polyethylene glycol 3350