7Y16

Crystal structure of rRNA-processing protein Las1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	51.495, 58.975, 158.688
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.980 - 1.800
R-factor	0.213
Rwork	0.212
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6of4
RMSD bond length	0.015
RMSD bond angle	1.500
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rpim	0.033	0.305
Number of reflections	44296	2147
<I/σ(I)>	22
Completeness [%]	97.7
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Tris-HCl, pH 8.0, 0.2 M MgCl2, and 25% PEG 3350