7Y10

Crystal structure of AtSFH5-Sec14 in complex with DPPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-09
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	99.693, 114.551, 111.836
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.440 - 2.100
R-factor	0.2262
Rwork	0.225
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aua
RMSD bond length	0.010
RMSD bond angle	1.497
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.157	0.837
Rmeas	0.171	0.933
Rpim	0.067	0.402
Number of reflections	37354	3480
<I/σ(I)>	11.9	1.84
Completeness [%]	99.2	93.4
Redundancy	6.4	5
CC(1/2)	0.991	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.1 M MES pH 6.0, 20% PEG6000, 0.2 M NaCl