7Y0Z

Crystal structure of Pseudomonas aeruginosa PvrA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	C 2 2 21
Unit cell lengths	93.734, 96.418, 104.911
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.490 - 2.300
R-factor	0.206
Rwork	0.201
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7y0y
RMSD bond length	0.008
RMSD bond angle	0.922
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rpim	0.048	0.432
Number of reflections	21479	1072
<I/σ(I)>	19.1
Completeness [%]	100.0
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10% w/v polyethylene glycol (PEG) 1000, 10% w/v PEG 8000