7XZF
Wild type of the N-terminal domain of fucoidan lyase FdlA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 193.15 |
| Detector technology | PIXEL |
| Collection date | 2017-12-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.001, 58.172, 88.319 |
| Unit cell angles | 90.27, 101.60, 92.01 |
Refinement procedure
| Resolution | 24.990 - 1.300 |
| R-factor | 0.1369 |
| Rwork | 0.136 |
| R-free | 0.15760 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.405 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmeas | 0.113 | |
| Rpim | 0.505 | |
| Number of reflections | 290039 | 13327 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 94.8 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1M CAPS pH 10.5, 0.2M Lithium sulfate, 2M ammonium sulfate |
