7XZE

Mutant (H176A) of the N-terminal domain of fucoidan lyase FdlA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2018-12-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	54.017, 58.179, 88.537
Unit cell angles	90.27, 101.41, 92.19

Refinement procedure

Resolution	52.910 - 1.800
R-factor	0.1596
Rwork	0.158
R-free	0.18940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xzf
RMSD bond length	0.014
RMSD bond angle	1.120
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.000	1.860
High resolution limit [Å]	1.800	1.800
Rmeas	0.070	0.910
Number of reflections	91221	6539
<I/σ(I)>	24.3
Completeness [%]	92.5
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1M CAPS, pH10.5, 0.2M lithium sulfate, 2M ammonium sulfate