7XZD
Mutant (F179A) of the N-terminal domain of fucoidan lyase FdlA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 193.15 |
| Detector technology | PIXEL |
| Collection date | 2021-10-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.009, 58.000, 88.501 |
| Unit cell angles | 89.98, 101.39, 92.05 |
Refinement procedure
| Resolution | 27.790 - 1.750 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7xzf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.068 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmeas | 0.088 | 0.370 |
| Number of reflections | 112145 | 10128 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 71.1 | |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 291.15 | 0.1 M CAPS pH 10.5, 0.2 M lithium sulfate, 2 M ammonium sulfate |
